Compound comparison
BPC-157 vs TB-500 · Reference Compound Comparison
Comparison of two of the most commonly co-investigated peptide reference compounds in tissue-research protocols. Both are sold by ZynoPep as ≥99% purity lyophilized standards with full COA.
Shared characteristics
- ·Both are synthetic linear peptides with no disulfide bridges
- ·Both supplied as lyophilized powders, sealed under nitrogen
- ·Both reconstitute readily in bacteriostatic water at >10 mg/mL
- ·Both ship with batch-specific Certificate of Analysis (HPLC + ESI-MS)
- ·Both qualified at ≥99% purity by reversed-phase HPLC
Side-by-side differences
| Aspect | BPC-157 | TB-500 |
|---|---|---|
| Peptide length | 15 residues | 43 residues (acetylated) |
| Molecular weight | 1419.55 g/mol | 4963.44 g/mol |
| Molecular formula | C62H98N16O22 | C212H350N56O78S |
| CAS number | 137525-51-0 | 77591-33-4 |
| Origin | Synthetic pentadecapeptide modeled on BPC fragment | Synthetic fragment of thymosin β-4 |
| Key labile residues | Asp10, Asp11 (isomerization) | Met6 (oxidation), N-terminal Ac group |
| Sulfur content | None | Single methionine |
| Reconstituted half-life at 25 °C | ~14 days | ~7 days (Met oxidation) |
When researchers choose each
BPC-157 is selected for protocols studying short peptide stability and proline-rich backbone analytics. TB-500 is selected for protocols on larger acetylated peptide fragments where N-terminal blocking and methionine oxidation kinetics are research variables. The two are frequently co-studied in tissue-research literature; many laboratories order both alongside the GLOW reference blend.
Order
Pentadecapeptide BPC-157
10mg vial · ≥99% purity
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Order
Thymosin β-4 fragment (TB-500)
10mg vial · ≥99% purity
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Frequently asked questions
What is the main difference between BPC-157 and TB-500?
Size and origin. BPC-157 is a 15-residue synthetic pentadecapeptide (1419.55 g/mol, CAS 137525-51-0) with a proline-rich, protease-resistant backbone. TB-500 is a 43-residue, N-terminally acetylated synthetic fragment of thymosin β-4 (4963.44 g/mol, CAS 77591-33-4) containing a single methionine. They also degrade by different pathways.
Can you stack BPC-157 and TB-500?
In a research-compound context the two are commonly co-investigated together, an informal pairing sometimes called the “Wolverine Stack.” ZynoPep supplies the two plus GHK-Cu as the single-vial GLOW reference blend. This describes co-presence of reference materials for in-vitro protocols only and implies no combined biological effect in any organism.
How do BPC-157 and TB-500 differ in stability and half-life?
BPC-157's labile sites are Asp10/Asp11 (isomerization), giving a reconstituted working half-life near 14 days at 25 °C. TB-500's vulnerable residue is Met6 (oxidation to sulfoxide), giving a shorter practical half-life of roughly 7 days at 25 °C in air-saturated solution. Both are most stable lyophilized at −20 °C and aliquoted at −80 °C once reconstituted.
Is BPC-157 or TB-500 better?
Neither is better in a general sense; they answer different research questions. BPC-157 suits protocols centered on short, proline-rich peptide analytics and proteolytic stability, while TB-500 suits work involving a larger acetylated fragment and methionine-oxidation kinetics. Selection should follow the specific model system or analytical requirement.
Research overview