GLOW Blend Storage, Reconstitution & Cu Complex Stability

Sealed lyophilized blend: −20 °C, protected from light. The Cu(II) center is photo- and oxygen-sensitive in solution but stable in the lyophilized state for 24+ months.

Reconstitute the 70 mg blend in 3-5 mL bacteriostatic water. Expect a clear, faintly blue solution from the Cu(II)-glycyl-histidyl-lysine coordination complex. Do not use chelating buffers (EDTA, citrate at high concentration) — they will displace the copper center.

At 2-8 °C in bacteriostatic water, the reconstituted blend retains characteristic blue color and >90% intact GHK-Cu coordination for 14 days. Color fading toward green or grey indicates Cu(II) reduction or chelation. The BPC-157 and TB-500 components follow their individual stability profiles (see their respective handling guides).

Single freeze-thaw is well-tolerated. Multiple cycles can cause partial Cu dissociation due to ice-induced concentration gradients. Aliquot once and freeze at −80 °C.

TB-500 in this blend will show the same methionine oxidation susceptibility as the standalone product — store reconstituted aliquots cold and protected from air. BPC-157 in this blend is subject to Asp isomerization on extended storage above pH 7.

COA documents the three components individually with their HPLC purity, MS identity, and blend mass uniformity.