Chemistry Data Sheet
TB-500 (Thymosin β-4 Fragment) Data Sheet · CAS 77591-33-4
Full analytical specification for our TB-500 lyophilized reference standard, a synthetic acetylated 43-amino-acid fragment of thymosin β-4 used as a reference compound in in-vitro research. Synthesized & packaged in the USA.
CAS
77591-33-4
Formula
C212H350N56O78S
MW
4963.44 g/mol
Purity
≥99%
Identity & Nomenclature
TB-500 is the synthetic acetylated N-terminal 43-residue fragment of thymosin β-4. Trade name: TB-500. CAS registry number: 77591-33-4. Sometimes annotated as Tβ4 (1-43) or thymosin beta-4 acetate fragment.
Molecular Formula & Mass
Molecular formula: C212H350N56O78S. Monoisotopic mass: 4960.4920 Da. Average molecular weight: 4963.44 g/mol. Contains one methionine sulfur. Calculated isoelectric point (pI): ~5.1.
Sequence
Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser (acetylated at the N-terminus).
Purity Specification
≥99% by reversed-phase HPLC at 220 nm. Mass confirmation by ESI-MS or MALDI-TOF; theoretical [M+H]+ at m/z 4964.4. Peptide content quantified by amino acid analysis (AAA) and corrected for counterion mass.
Physical Form & Storage
Lyophilized white powder. Highly water-soluble (>20 mg/mL). Store sealed at −20 °C protected from light and moisture. Solution-state stability is lower than lyophilized; reconstituted aliquots should be stored at −80 °C and freeze-thaw cycles minimized.
Certificate of Analysis
Shipment includes a batch-specific COA documenting HPLC purity (≥99% at 220 nm), MS identity confirmation, water content (Karl Fischer), endotoxin, and net peptide content.
Order this reference compound
Thymosin β-4 fragment (TB-500)
10mg vial · ≥99% purity · COA included
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