GLP3 Storage, Reconstitution & Stability Reference

Sealed lyophilized GLP3: −20 °C, protected from light and moisture. Stable >24 months. The hygroscopic powder should be brought to room temperature in its sealed vial before opening.

GLP3 is amphipathic with limited solubility in pure water at neutral pH. Standard protocol: reconstitute 10 mg in 1 mL of 0.1% NaOH (to dissolve), then dilute 1:10 in bacteriostatic water or phosphate buffer (pH 7.4) to achieve a 1 mg/mL working stock. Alternative: 0.1% acetic acid then dilute into PBS — both yield clear solutions.

Reconstituted at 1 mg/mL in PBS pH 7.4 at 2-8 °C: >95% intact for 21 days. At pH <5 or >9, increased aspartyl isomerization and hydrolysis. The Aib residues (positions 2, 16, 20, 24) confer increased α-helical stability vs. native GLP-1 backbones, reducing aggregation propensity in solution.

For long-term work, aliquot reconstituted stock into single-use volumes and store at −80 °C. Avoid >3 freeze-thaw cycles.

GLP3 self-associates above ~5 mg/mL in physiologic buffer; working concentrations below 1 mg/mL minimize aggregation. Dynamic light scattering may be used to confirm monodispersity in critical preparations.

Detailed forced-degradation HPLC profiles available on request for institutional buyers. COA ships with every vial.